Technical Bulletin: CELLvo™ Human Articular Chondrocytes Exhibit Native Chondrocyte Phenotype

Technical Bulletin: CELLvo™ Human Articular Chondrocytes Exhibit Native Chondrocyte Phenotype

CELLvo™ Matrix has recently been shown to improve the isolation and expansion of human chondrocytes, while minimizing significant change in phenotype [1]. Here, we compare gene expression of CELLvo™ Human Articular Chondrocytes (HC-A) to other commercially available articular chondrocyte products. Additionally, we examine the effect of culture on substrate choice (CELLvo™ Chondrocyte-Derived (CD) Matrix (in development) vs. tissue culture plastic (TCP)) on chondrocyte gene expression. We show that CELLvo™ HC-A are the only chondrocytes with a native phenotype, and that CELLvo™ CD Matrix minimizes loss of phenotype during in vitro expansion.